HistaSure™ ELISA High Sensitive

HistaSure™ ELISA High Sensitive

This assay measures Histamine quantitatively in each kind of fish and fish products such as fresh fish (tuna, anchovy, mackerel, herring, salmon;), canned fish, salted fish, fish in oil, and fish meal (click here for an overview).

Quality control measures designed to minimize the occurrence of scombrotoxic fish require the determination of histamine levels in the range of approximately 10 to 200 ppm. Good quality fish contain less than 10 ppm histamine, a level of 30 ppm indicates significant deterioration, and 50 ppm is considered to be evidence of definite decomposition. The defect action level (DAL), the level at which regulatory actions are taken for histamine, is 50 ppm (P. L. Rogers, W. F. Staruszkiewicz, Journal of Aquatic Food Product Technology, Vol. 9 (2) 2000 p. 5 - 17

In a comparative study where different commercial Histamine test kits had been compared the HistaSure™ ELISA High-Sensitive showed the best correlation to the HPLC-method and the authors concluded that this kit “is found to be a reliable method for testing histamine in TFPs (traditional food products) either for regulatory or HACCP applications in monitoring such hazard” [Sevim Köse et al., Commercial test kits and the determination of histamine in traditional (ethnic) fish products-evaluation against an EU accepted HPLC method; Food Chemistry Volume 125, Issue 4, 15 April 2011, Pages 1490–1497; please click here to view the complete publication].

Catalog Nr.: 
FC E-3100
Name: 

HistaSure™ ELISA High Sensitive

Description: 
Enzyme immunoassay (ELISA) for the quantitative determination of Histamine in food
Design: 
Acylation in 96-well acylation plate. 6 standards.2 controls. 12x8 break apart wells microtiter plate.
Sample Volume: 
1g fish meal; 10g fresh fish. sausage. cheese; 10 µl milk; 20 µl wine
Total Assay Time: 
Acylation 15 min and ELISA 1 hour
Standard Range: 

depends on kind of sample

Sensitivity: 

0.15 µg/l

Storage: 
2 - 8 °C
Kit: 
96 Determinations
Advantages: 
  • Very short assay times
  • Quantitative determination in an easy to use ELISA
  • Easy sample preparation
  • Small sample volumes
  • High correlation with HPLC

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